含义Meaning
抑制剂只结合 ES 复合物：\(V_{\max}\) 与 \(K_\mathrm{M}\) 按同一比例 \(1/\alpha'\) 降低，双倒数图为平行线。
The inhibitor binds only ES: \(V_{\max}\) and \(K_\mathrm{M}\) both fall by \(1/\alpha'\); Lineweaver–Burk lines are parallel.
符号说明Symbols
| 符号Symbol | 含义 Meaning | 单位 Unit |
|---|---|---|
| \(\alpha'\) | 1 + [I]/K_I′1 + [I]/K_I′ | 1 |
推导Derivation
只有 \(\mathrm{ES} + \mathrm{I} \rightleftharpoons \mathrm{ESI}\)。守恒 \([\mathrm{E}]_0 = [\mathrm{E}] + \alpha'[\mathrm{ES}]\)。
Only \(\mathrm{ES} + \mathrm{I} \rightleftharpoons \mathrm{ESI}\); conservation \([\mathrm{E}]_0 = [\mathrm{E}] + \alpha'[\mathrm{ES}]\).
解出 \(v_0\)；双倒数形式 \(1/v_0 = (K_\mathrm{M}/V_{\max})(1/[\mathrm{S}]) + \alpha'/V_{\max}\)，斜率不变。
Solving gives \(v_0\); the reciprocal form has unchanged slope.
适用条件与局限Assumptions & limitations
单底物酶中少见，多见于多底物酶的某一底物。
Rare for one-substrate enzymes; common for one substrate of multi-substrate enzymes.
例题Worked example
锂离子对肌醇单磷酸酶的抑制是经典的反竞争性抑制。
Li⁺ inhibition of inositol monophosphatase is a classic uncompetitive case.