定义Definition
移液管是用于准确移取一定体积液体的玻璃量器。中间有膨大部分、只有一条标线的称为大肚移液管(单标线移液管),用于移取固定体积;带有分刻度的直管称为吸量管,可移取不同体积。微量移液器(移液枪)用于微升级液体。
Pipettes are glass instruments for transferring accurately measured volumes of liquid. A volumetric (bulb) pipette with one mark delivers a fixed volume; a graduated (measuring) pipette delivers variable volumes. Micropipettes handle microlitre volumes.
使用方法How to use it
用洗耳球(绝不可用嘴吸)吸取溶液至标线以上,先用待取液润洗 2–3 次;吸液后用食指按住管口,擦干外壁,缓慢放液使弯月面最低点与标线相切;将移液管尖端靠在接收容器内壁,垂直放液,流完后停留约 15 秒。标有“吹”字的移液管需把尖端残留液吹出,未标的则不吹。
Draw liquid above the mark with a pipette bulb, never by mouth, after rinsing two or three times with the solution; cap with the forefinger, wipe the outside, and let liquid out slowly until the bottom of the meniscus touches the mark; then deliver with the tip against the inner wall of the receiver, held vertical, and wait about 15 seconds after it drains. Only pipettes marked 'blow-out' should have the last drop blown out.
精度Accuracy
A 级 25 mL 单标线移液管允差 ±0.03 mL。移液管是“量出式”量器(标 Ex),而容量瓶是“量入式”(标 In)。微量移液器利用活塞排出空气产生负压吸液,量程 0.1 μL–10 mL,需定期校准,吸头一次性使用以避免交叉污染。
A class-A 25 mL volumetric pipette is accurate to ±0.03 mL. Pipettes are calibrated to deliver ('Ex'), whereas volumetric flasks are calibrated to contain ('In'). Micropipettes draw liquid by piston displacement over ranges from 0.1 μL to 10 mL, need regular calibration, and use disposable tips to avoid cross-contamination.
由来Origin
1824 年盖-吕萨克在容量分析中使用并命名了移液管(pipette,源自法语“小管子”)。1957 年德国马堡大学的海因里希·施尼特格发明了现代按钮式微量移液器,1960 年代由埃彭道夫公司商业化。
Gay-Lussac used and named the pipette (French for 'little pipe') in volumetric analysis in 1824. Heinrich Schnitger at the University of Marburg invented the modern push-button micropipette in 1957, and Eppendorf commercialised it in the 1960s.
年表Timeline
- 1824
盖-吕萨克命名 pipette
Gay-Lussac names the pipette
- 1957
施尼特格发明微量移液器
Schnitger invents the micropipette